Have you also attempted to process frozen tissue samples as the teacher or the student who has conducted ATAC-seq library building experiments? Have you ever encountered issues with poor tissue sample lysis efficiency? Have you ever experienced suboptimal TSS enrichment? Have you ever encountered excessive background noise? To address the above-mentioned issues and make ATAC-seq experiments simpler and more efficient, Wuhan Yingzi Gene Technology Co., Ltd. has independently developed an ATAC-seq library building kit.

Yingzi Gene ATAC-seq Library Building Kit Diagram 

1. Product Features

1.1 Suitable for Various Sample Types,  Perfectly Compatible with Frozen Tissue, Low-Input Frozen Tissue, Fresh Tissue Samples, and Cell Samples.

1.2 Simple and Efficient, Rapid Isolation of Nuclei from Tissue Samples, Library Preparation Completed in Just 4 Hours. 

1.3 Outstanding Performance, High Signal-to-Noise Ratio, Excellent Reproducibility, and Excellent Results Achievable with Input as Low as 100 cells. 

2. Performance Results Display 

2.1 Perfect Adaptation to Frozen Tissue Sample Library Building

Yingzi Gene used its self-developed ATAC-seq Library Building Kit to construct ATAC libraries from various types of frozen tissue samples. The results indicate that the Yingzi Gene ATAC-seq Library Building Kit consistently produces stable libraries with typical characteristic peaks in the library profiles.

2.2 Simple and Efficient, Rapid Nuclei Isolation from Tissue Samples 

Yingzi Gene used its self-developed ATAC-seq Library Building Kit to construct ATAC libraries, enabling efficient nuclei extraction from tissue samples. The results indicate that the Yingzi Gene ATAC-seq Library Preparation Kit provides excellent nuclear extraction efficiency.

Nuclear microscopy results of porcine hypothalamus cleavage extraction

Nuclear microscopy results of porcine cerebellar cleavage extraction

Note: Nuclei are well dispersed with clear and smooth boundaries, exhibiting a regular circular shape, deep staining, and relatively dense nuclear status, and indicating excellent nuclear extraction efficiency 

2.3 Outstanding Performance, Excellent Results Achieved with as Few as 100 Cells

Yingzi Gene used its self-developed ATAC-seq Library Building Kit to construct ATAC libraries with different cell inputs. The results indicate that even with sample sizes as low as 100 cells, the quality of downstream data remains excellent, and the TSS enrichment results demonstrate significant enrichment at transcription start sites. IGV visualization results show a high consistency in peaks between 100 cells, 500 cells, and 50,000 cells.

Caption: TSS Enrichment Results at Different Cell Input Levels 

3. Scope of Application

Suitable for ATAC Library Building in Animal Fresh Tissues, Frozen Tissues, and Cells.