ChIP-seq

I. Product Introduction

Chromatin Immunoprecipitation (ChIP) is a classic technology for studying the interaction between proteins and DNA, and widely used in research on genomic transcription factor binding characteristics, histone modification features, etc. This technology, when combined with next-generation sequencing, forms the ChIP-seq technology, which is extensively applied in functional genomics research of animals, plants, and humans. It has revealed the specific interactions between proteins and genomic DNA and provided insights into the regulation of gene expression.

II Service Process

1. Suitable for various tissue and cell samples: Fresh and frozen tissues are both acceptable, and various types of cells can be used.

2. Low Initial Sample Size: The minimum quantity for tissue samples is 1g/sample, and for cells, it is 5x10^7 cells/sample.

3. High Data Quality: Quality control results, such as FRiP and TSS enrichment analysis, meet the standards set by the ENCODE project.

In November 2020, Hongbo Yang et al. from Northwestern University in the United States published the results of research on zebrafish genome regulatory elements in Nature magazine, and identified the promoter and enhancer elements of zebrafish genome through the hiP-seq data of H3K4me3 and H3K27a. The heterochromatin regions of zebrafish were identified by ChiP-seq data of H3K9me3 and H3K9me2 factors. In addition, the zebrafish genome was comprehensively annotated through multi-omics integration analysis.

Figure 3. Identification of whole-genome regulatory elements in zebrafish

Literature： A map of cis-regulatory elements and 3D genome structures in zebrafish, 2020, Nature

In February 2020, Zhou et al. from Huazhong Agricultural University published their research on miRNA Regulation of Myoblast Proliferation and Differentiation in the Journal Cell Death & Disease. The study integrated miRNA sequencing, transcription factor ChIP-seq, and ATAC-seq data to analyze the regulatory patterns of miRNA expression during the process of myoblast proliferation and differentiation. The results revealed that miRNA expression is regulated by chromatin open state during the process of myoblast differentiation.

Above: Integrated multi-omics data to analyze the molecular mechanism of miRNA expression regulation during the proliferation and differentiation of myoblasts

Literature： Chromatin accessibility is associated with the changed expression of miRNAs that target members of the Hippo pathway during myoblast differentiation, 2020, Cell death & disease

1. Sample Types and Sample Amounts

Fresh tissues, frozen tissues, fresh cells, frozen cells, and IP-enriched DNA samples are all accepted. Please inquire for other types of samples.

The minimum quantity for cell samples is 5x10^7 cells/sample, for tissue samples, it is 1g/sample, and for IP-enriched DNA samples, it is 10ng/sample.

2. Sampling Considerations

(1) Repetition Control: Sampling should strive to maintain consistent conditions such as time, location, gender, and age, etc.

(2) Avoid Cross-Contamination: During the sampling process, efforts should be made to prevent cross-contamination between samples.

3. Sample Preservation

(1) Tissue Samples: After animal euthanasia, collect the tissues in30 minutes. After simple dissection, place the tissues in 2ml cryovials and freeze the rapidly in liquid nitrogen for a minimum of 2 hours, and then transfer them to a -80°C freezer for storage. Sampling should be kept at low temperatures, and the procedures should be performed rapidly to avoid sample degradation (please inquire for specific details).

(2) Cell Samples: Fix with 1% formaldehyde (please inquire for specific details).