Epigenetic Service

Epigenetic

Epigenetics refers to the phenomenon of genetic variation caused solely by changes in chromatin state without altering the DNA sequence. In the genomes of animals and plants, non-coding DNA sequences make up more than 90%, and previous research considered these non-coding sequences as "junk DNA". With the advancement of scientific technology, recent studies have shown that these non-coding sequences are DNA elements with significant regulatory functions. These DNA elements can alter chromatin spatial conformation and the binding status of transcriptional regulators, and regulate gene spatio-temporal expression through epigenetic modifications, thereby resulting in phenotypic variations in animals and plants, as well as the occurrence of human diseases, etc. Therefore, through epigenetic research, it is possible to gain a more precise understanding of the molecular mechanisms of biological phenomena such as phenotypic changes in animals and plants and the occurrence of human diseases, etc. The contents of epigenetic research include DNA methylation (1D), protein-DNA interactions (2D), chromatin long range interactionss (3D), and RNA methylation (m6A), etc.

ATAC-seq

ATAC-seq is to add Tn5 transposase in living cells or quick frozen tissues to cleave open nuclear chromatin region within the cell nucleus, and to study chromatin accessibility in conjunction with high-throughput sequencing technology. This technology can identify open chromatin regions at the whole-genome level.

CUT&Tag

CUT&Tag is a novel technology for in situ studying protein-DNA interactions, which is to achieve targeted cleavage and sequencing adapter insertion through the binding of Protein G/A-tn5 to specific proteins on the genome, and to obtain information of protein-DNA interactions through sequencing. This technology can replace ChIP-seq to a certain extent.

ChIP-seq

ChIP-seq is a classic technology used to study protein-DNA interactions and allows for comprehensive analysis of interactions between proteins and DNA at the whole-genome level. It is widely used in the study of transcription factor binding sites or histone modification sites.

Hi-C

Hi-C is a novel technology used for studying the 3D spatial structure of the genome, which is able to clearly resolve 3D remote interaction information, including chromatin A/B territory, TAD and loops,etc., and to elucidate regulatory elements and their remote interactions in combination with epigenetic data including ChIP-seq and ATAC-seq, etc.

HiChIP/ChIA-PET

HiChIP and ChIA-PET are both new technologies used to study specific protein-mediated genomic 3D structures. Its principle is to conduct in situ enzymatic cleavage and ligation within the cell nucleus, and achieve the precise capture of specific protein-mediated genomic remote interactions in combination with ChIP experiments, both of which feature high resolution.

Capture Hi-C

The Capture Hi-C technology is the capturing process of adding a specific probe to the Hi-C library. The principle is to design a probe for the target region, and then use it to capture the desired fragments from a standard Hi-C library for sequencing. The advantage of this technology is its ability to resolve the remote interactions of a specific region.

GRID-seq

GRID-seq is a novel genome-wide method for analyzing DNA-RNA interactions, which is to identify RNAs involved in transcriptional regulation at the whole-genome level and to decipher the functions of lncRNAs at a whole-genome level.

DNA Methylation

DNA methylation is a chemical modification that occurs on a DNA sequence, and can influence gene expression without altering the DNA sequence. It is possible to identify genomic methylation loci by utilizing bisulfite treatment in combination with high-throughput sequencing methods.